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Titolo Development and validation of an HPLC method to quantify 3,4-dideoxyglucosone-3-ene in peritoneal dialysis fluids
Autore Frischmann M, Spitzer J, F├╝nfrocken M, Mittelmaier S, Deckert M, Fichert T, Pischetsrieder M. - Department of Chemistry and Pharmacy, Food Chemistry, University of Erlangen-Nuremberg, Schuhstr. 19, 91052 Erlangen, Germany
Referenza Biomedical Chromatography 2009; DOI 10.1002/bmc.1194
Contenuto A method was developed and validated to quantify 3,4-dideoxyglucosone-3-ene in peritoneal dialysis fluids by high-performance liquid chromatography with UV detection after derivatization with o-phenylenediamine. The advantages of this method compared with direct HPLC analysis are (i) the possibility of quantifying 3,4-dideoxyglucosone-3-ene simultaneously together with other glucose degradation products, (ii) the compatibility of the method with MS detection for unequivocal identification of the analyte and (iii) a bathochromic shift of the UV absorbance maximum which leads to higher selectivity. The validated method was used to measure 3,4-dideoxyglucosone-3-ene concentrations additionally to the glucose degradation products 3-deoxyglucosone, methylglyoxal, glyoxal, 5-hydroxymethylfurfural, 2-furaldehyde, formaldehyde and acetaldehyde in 19 commercial products for peritoneal dialysis. Copyright (c) 2009 John Wiley & Sons, Ltd.
Data 25.05.2009
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