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Titolo Isolation and Characterization of Resident Mesenchymal Stem Cells in Human Glomeruli
Autore Stefania Bruno,* Benedetta Bussolati,* Cristina Grange, Federica Collino, Ludovica Verdun di Cantogno, Maria Beatriz Herrera, Luigi Biancone, Ciro Tetta, Giuseppe Segoloni, and Giovanni Camusi - Department of Internal Medicine, Molecular Biotechnology Center and Research Center for Experimental Medicine (CeRMS), University of Torino, Torino, Italy and Fresenius Medical Care, Bad Homburg, Germany. - *These authors contributed equally to this work.
Referenza STEM CELLS AND DEVELOPMENT 2009; DOI: 10.1089/scd.2008.0320
Contenuto In human, renal resident stem cells were identifi ed within the interstitium, the tubular cells, and the Bowman?s capsule. The aim of the present study was to investigate whether multipotent stem cell are present also in the adult human-decapsulated glomeruli and whether they represent a resident population. We found that human glomeruli deprived of the Bowman?s capsule contain a population of CD133+CD146+ cells and a population of CD133-CD146+ cells expressing mesenchymal stem cell (MSC) markers and renal stem cell markers CD24 and Pax-2. The CD133+CD146+ cells differed from those previously isolated from Bowman?s capsule as they coexpressed endothelial markers, such as CD31 and von Willebrand factor (vWF), were CD24-negative and were not clonogenic, suggesting an endothelial commitment. The glomerular mesenchymal CD133-CD146+ population (Gl-MSC) exhibited self-renewal capability, clonogenicity, and multipotency. Beside osteogenic, adipogenic, and chondrogenic differentiation, these cells were able to differentiate to endothelial cells and epithelial cells expressing podocytes markers such as nephrin, podocin, and synaptopodin. Moreover, Gl-MSC when cultured in appropriate conditions, acquired mesangial cell markers such as alpha-smooth muscle actin (α-SMA) and angiotensin II (AT-II) receptor I. The expression of the embryonic organ-specifi c PAX-2 gene and protein and of donor sex identity when isolated from glomeruli of a renal allograft suggested these cells to be a tissue resident population. In conclusion, these results indicate the presence of a multipotent mesenchymal cell population resident in human glomeruli that may have a role in the physiological cell turnover and/or in response to glomerular injury.
Data 13.03.2009
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