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Titolo ASN 2005 - [PUB363] Protein Glycation, Oxidation and Nitration Residues in Plasma Proteins and Free Adducts in Uremia: Effect of Hemodiafiltration and Hemodialysis.
Autore Paul J. Thornalley, Nicole Schupp, Naila Ahmed, Johann Braun, Joerg Vienken, Helga Stopper, August Heidland Department of Biological Sciences, University of Essex, Colchester, Essex, United Kingdom; Institute of Pharmacology and Toxicology, University of Wuerzburg, Wuerzburg, Germany; Kuratorium for Dialysis and Kidney Transplantation, University of Erlangen-Nurnberg, Nurnberg, Germany; BioSciences Department, Fresenius Medical Care, Bad Homburg, Germany; Department of Internal Medicine, University of Wuerzburg, Wuerzburg, Germany
Referenza ASN 2005 - 8.11.2005 - 13.11.2005 Philadelphia, Pennsylvania, USA
Contenuto The aim of this study was to assess if high flux hemodialysis (HD) and hemodiafiltration (HDF) eliminated protein glycation, oxidation and nitration adducts effectively in end-stage renal disease (ESRD). Protein glycation, oxidation and nitration adducts were quantified in a cross-sectional study of two groups of ten ESRD patients on HD and HDF therapy, compared with 20 normal healthy controls. The concentrations of 9 glycation adducts, the oxidation adduct methionine sulfoxide (MetSO), and the nitration adduct 3-nitrotyrosine (3-NT) were determined by liquid chromatography with tandem mass spectrometry. Adduct residues were determined in plasma protein fractionated by ultrafiltration (molecular mass cut-off: 12 kDa) and free adducts were determined in plasma ultrafiltrate. In plasma protein, fructosyl-lysine (FL) residues were decreased 38% and 42% in HDF and HD patients, respectively, whereas advanced glycation endproduct (AGE) residues were increased 2 5 fold, with respect to controls. MetSO residues were increased 4-fold and 3-NT residues 6 8-fold in HDF and HD patients. HDF patients had slightly higher levels of methylglyoxal-derived hydroimidazolone and 3-NT residues than HD patients. In both HDF and HD patients, FL and 3-NT free adducts were increased 2-fold whereas AGE free adducts were increased 4 148 fold; HDF patients had a slightly higher concentration of FL free adduct and a lower concentration of N-carboxyethyl-lysine (CEL) free adduct. HD and HDF patients therefore had increased concentrations of plasma protein glycation, oxidation and nitration adduct residues and particularly increased concentrations of related free adducts compared to normal controls.
Data 20.12.2005
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